Journal: The EMBO Journal
Article Title: GNAS/PKA signaling promotes aberrant osteochondral differentiation of Gli1 + tendon sheath progenitors
doi: 10.1038/s44318-025-00553-7
Figure Lengend Snippet: ( A ) GO analysis of DEG between clusters 5 and 8. ( B ) Heatmap of DEG in clusters 5 and 8. ( C ) Feature plot showing the expression of Gnas in mesenchymal and tendon lineage cells. ( D ) Representative immunofluorescence images of GNAS expression in uninjured tendon and injured site of Gli1 - Cre ERT2 ; Ai9 mice at 7, 21, and 63 dpi. Scale bar, 50 μm. ( E , F ) Statistical analysis of GNAS + region ( E ) and GNAS + /GLI1 + region ( F ) in uninjured tendon and injured site of Gli1 - Cre ERT2 ; Ai9 mice at 7, 21, and 63 dpi ( n = 5 per group). P values in ( E ): 7 dpi vs uninjured **** P = 9.47e-11. 21 dpi vs uninjured **** P = 2.03e-8, 63 dpi vs uninjured **** P = 3.21e-5. P values in ( F ): 7 dpi vs uninjured **** P = 2.64e-6, 21 dpi vs uninjured **** P = 3.64e-6, 63 dpi vs uninjured **** P = 9.58e-5. ( G, H ) Statistical analysis of p-PKA substrate + region ( G ) and p-PKA substrate + /GLI1 + region ( H ) in uninjured tendon and injured site of Gli1 - Cre ERT2 ; Ai9 mice at 7, 21, and 63 dpi ( n = 5 per group). P values in ( G ): 7 dpi vs uninjured **** P = 6.33e-6, 21 dpi vs uninjured *** P = 1.15e-4, 63 dpi vs uninjured * P = 1.17e-2. P values in ( H ): 7 dpi vs uninjured **** P = 1.96e-5, 21 dpi vs uninjured *** P = 2.63e-3, 63 dpi vs uninjured **** P = 6.44e-4. ( I – L ) Representative immunofluorescence images of p-PKA substrate expression in uninjured tendon ( I ) and injured site of Gli1 - Cre ERT2 ; Ai9 mice at 7 ( J ), 21 ( K ), and 63 dpi ( L ). Scale bar, 50 μm. Data are presented as mean ± SD. All P values were determined by unpaired Student’s t test.
Article Snippet: Anti-mouse GNAS , Proteintech , Cat#10150-2-AP.
Techniques: Expressing, Immunofluorescence