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biotinylated galanthus nivalis agglutinin biotin gna  (Vector Laboratories)


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    Vector Laboratories biotinylated galanthus nivalis agglutinin biotin gna
    Biotinylated Galanthus Nivalis Agglutinin Biotin Gna, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 66 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated galanthus nivalis agglutinin biotin gna/product/Vector Laboratories
    Average 93 stars, based on 66 article reviews
    biotinylated galanthus nivalis agglutinin biotin gna - by Bioz Stars, 2026-03
    93/100 stars

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    ( A ) GO analysis of DEG between clusters 5 and 8. ( B ) Heatmap of DEG in clusters 5 and 8. ( C ) Feature plot showing the expression of <t>Gnas</t> in mesenchymal and tendon lineage cells. ( D ) Representative immunofluorescence images of GNAS expression in uninjured tendon and injured site of Gli1 - Cre ERT2 ; Ai9 mice at 7, 21, and 63 dpi. Scale bar, 50 μm. ( E , F ) Statistical analysis of GNAS + region ( E ) and GNAS + /GLI1 + region ( F ) in uninjured tendon and injured site of Gli1 - Cre ERT2 ; Ai9 mice at 7, 21, and 63 dpi ( n = 5 per group). P values in ( E ): 7 dpi vs uninjured **** P = 9.47e-11. 21 dpi vs uninjured **** P = 2.03e-8, 63 dpi vs uninjured **** P = 3.21e-5. P values in ( F ): 7 dpi vs uninjured **** P = 2.64e-6, 21 dpi vs uninjured **** P = 3.64e-6, 63 dpi vs uninjured **** P = 9.58e-5. ( G, H ) Statistical analysis of p-PKA substrate + region ( G ) and p-PKA substrate + /GLI1 + region ( H ) in uninjured tendon and injured site of Gli1 - Cre ERT2 ; Ai9 mice at 7, 21, and 63 dpi ( n = 5 per group). P values in ( G ): 7 dpi vs uninjured **** P = 6.33e-6, 21 dpi vs uninjured *** P = 1.15e-4, 63 dpi vs uninjured * P = 1.17e-2. P values in ( H ): 7 dpi vs uninjured **** P = 1.96e-5, 21 dpi vs uninjured *** P = 2.63e-3, 63 dpi vs uninjured **** P = 6.44e-4. ( I – L ) Representative immunofluorescence images of p-PKA substrate expression in uninjured tendon ( I ) and injured site of Gli1 - Cre ERT2 ; Ai9 mice at 7 ( J ), 21 ( K ), and 63 dpi ( L ). Scale bar, 50 μm. Data are presented as mean ± SD. All P values were determined by unpaired Student’s t test.
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    ( A ) GO analysis of DEG between clusters 5 and 8. ( B ) Heatmap of DEG in clusters 5 and 8. ( C ) Feature plot showing the expression of <t>Gnas</t> in mesenchymal and tendon lineage cells. ( D ) Representative immunofluorescence images of GNAS expression in uninjured tendon and injured site of Gli1 - Cre ERT2 ; Ai9 mice at 7, 21, and 63 dpi. Scale bar, 50 μm. ( E , F ) Statistical analysis of GNAS + region ( E ) and GNAS + /GLI1 + region ( F ) in uninjured tendon and injured site of Gli1 - Cre ERT2 ; Ai9 mice at 7, 21, and 63 dpi ( n = 5 per group). P values in ( E ): 7 dpi vs uninjured **** P = 9.47e-11. 21 dpi vs uninjured **** P = 2.03e-8, 63 dpi vs uninjured **** P = 3.21e-5. P values in ( F ): 7 dpi vs uninjured **** P = 2.64e-6, 21 dpi vs uninjured **** P = 3.64e-6, 63 dpi vs uninjured **** P = 9.58e-5. ( G, H ) Statistical analysis of p-PKA substrate + region ( G ) and p-PKA substrate + /GLI1 + region ( H ) in uninjured tendon and injured site of Gli1 - Cre ERT2 ; Ai9 mice at 7, 21, and 63 dpi ( n = 5 per group). P values in ( G ): 7 dpi vs uninjured **** P = 6.33e-6, 21 dpi vs uninjured *** P = 1.15e-4, 63 dpi vs uninjured * P = 1.17e-2. P values in ( H ): 7 dpi vs uninjured **** P = 1.96e-5, 21 dpi vs uninjured *** P = 2.63e-3, 63 dpi vs uninjured **** P = 6.44e-4. ( I – L ) Representative immunofluorescence images of p-PKA substrate expression in uninjured tendon ( I ) and injured site of Gli1 - Cre ERT2 ; Ai9 mice at 7 ( J ), 21 ( K ), and 63 dpi ( L ). Scale bar, 50 μm. Data are presented as mean ± SD. All P values were determined by unpaired Student’s t test.
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    Image Search Results


    ( A ) GO analysis of DEG between clusters 5 and 8. ( B ) Heatmap of DEG in clusters 5 and 8. ( C ) Feature plot showing the expression of Gnas in mesenchymal and tendon lineage cells. ( D ) Representative immunofluorescence images of GNAS expression in uninjured tendon and injured site of Gli1 - Cre ERT2 ; Ai9 mice at 7, 21, and 63 dpi. Scale bar, 50 μm. ( E , F ) Statistical analysis of GNAS + region ( E ) and GNAS + /GLI1 + region ( F ) in uninjured tendon and injured site of Gli1 - Cre ERT2 ; Ai9 mice at 7, 21, and 63 dpi ( n = 5 per group). P values in ( E ): 7 dpi vs uninjured **** P = 9.47e-11. 21 dpi vs uninjured **** P = 2.03e-8, 63 dpi vs uninjured **** P = 3.21e-5. P values in ( F ): 7 dpi vs uninjured **** P = 2.64e-6, 21 dpi vs uninjured **** P = 3.64e-6, 63 dpi vs uninjured **** P = 9.58e-5. ( G, H ) Statistical analysis of p-PKA substrate + region ( G ) and p-PKA substrate + /GLI1 + region ( H ) in uninjured tendon and injured site of Gli1 - Cre ERT2 ; Ai9 mice at 7, 21, and 63 dpi ( n = 5 per group). P values in ( G ): 7 dpi vs uninjured **** P = 6.33e-6, 21 dpi vs uninjured *** P = 1.15e-4, 63 dpi vs uninjured * P = 1.17e-2. P values in ( H ): 7 dpi vs uninjured **** P = 1.96e-5, 21 dpi vs uninjured *** P = 2.63e-3, 63 dpi vs uninjured **** P = 6.44e-4. ( I – L ) Representative immunofluorescence images of p-PKA substrate expression in uninjured tendon ( I ) and injured site of Gli1 - Cre ERT2 ; Ai9 mice at 7 ( J ), 21 ( K ), and 63 dpi ( L ). Scale bar, 50 μm. Data are presented as mean ± SD. All P values were determined by unpaired Student’s t test.

    Journal: The EMBO Journal

    Article Title: GNAS/PKA signaling promotes aberrant osteochondral differentiation of Gli1 + tendon sheath progenitors

    doi: 10.1038/s44318-025-00553-7

    Figure Lengend Snippet: ( A ) GO analysis of DEG between clusters 5 and 8. ( B ) Heatmap of DEG in clusters 5 and 8. ( C ) Feature plot showing the expression of Gnas in mesenchymal and tendon lineage cells. ( D ) Representative immunofluorescence images of GNAS expression in uninjured tendon and injured site of Gli1 - Cre ERT2 ; Ai9 mice at 7, 21, and 63 dpi. Scale bar, 50 μm. ( E , F ) Statistical analysis of GNAS + region ( E ) and GNAS + /GLI1 + region ( F ) in uninjured tendon and injured site of Gli1 - Cre ERT2 ; Ai9 mice at 7, 21, and 63 dpi ( n = 5 per group). P values in ( E ): 7 dpi vs uninjured **** P = 9.47e-11. 21 dpi vs uninjured **** P = 2.03e-8, 63 dpi vs uninjured **** P = 3.21e-5. P values in ( F ): 7 dpi vs uninjured **** P = 2.64e-6, 21 dpi vs uninjured **** P = 3.64e-6, 63 dpi vs uninjured **** P = 9.58e-5. ( G, H ) Statistical analysis of p-PKA substrate + region ( G ) and p-PKA substrate + /GLI1 + region ( H ) in uninjured tendon and injured site of Gli1 - Cre ERT2 ; Ai9 mice at 7, 21, and 63 dpi ( n = 5 per group). P values in ( G ): 7 dpi vs uninjured **** P = 6.33e-6, 21 dpi vs uninjured *** P = 1.15e-4, 63 dpi vs uninjured * P = 1.17e-2. P values in ( H ): 7 dpi vs uninjured **** P = 1.96e-5, 21 dpi vs uninjured *** P = 2.63e-3, 63 dpi vs uninjured **** P = 6.44e-4. ( I – L ) Representative immunofluorescence images of p-PKA substrate expression in uninjured tendon ( I ) and injured site of Gli1 - Cre ERT2 ; Ai9 mice at 7 ( J ), 21 ( K ), and 63 dpi ( L ). Scale bar, 50 μm. Data are presented as mean ± SD. All P values were determined by unpaired Student’s t test.

    Article Snippet: Anti-mouse GNAS , Proteintech , Cat#10150-2-AP.

    Techniques: Expressing, Immunofluorescence

    ( A – D ) Representative immunofluorescence images of GNAS expression in uninjured tendons ( A ) and injured sites of Gli1 - CreER T2 ; Ai9 mice at 5 ( B ), 7 ( C ) and 14 ( D ) dpi. Scale bar, 50 μm. ( E , F ) Statistical analysis of GNAS + region ( E ) and GNAS + /GLI1 + region ( F ) in uninjured tendons and injured sites of Gli1 - CreER T2 ; Acvr1 R206H/+ mice at 5, 7 and 14 dpi ( n = 5 per group). P values from left to right: **** P = 8.29e-10, **** P = 1.16e-8, **** P = 1.57e-8. **** P = 2.36e-9, **** P = 1.71e-6, **** P = 9.06e-6. ( G , H ) Statistical analysis of p-PKA substrate + region ( G ) and p-PKA substrate + /GLI1 + region ( H ) in uninjured tendons and injured sites of Gli1 - CreER T2 ; Acvr1 R206H/+ mice at 5, 7 and 14 dpi ( n = 5 per group). P values from left to right: **** P = 1.42e-6, **** P = 7.23e-6, **** P = 1.53e-5. **** P = 1.96e-5, ** P = 2.63e-3, **** P = 6.44e-4. ( I – L ) Representative immunofluorescence images of p-PKA substrate expression in uninjured tendons ( I ) and injured sites of Gli1 - CreER T2 ; Acvr1 R206H/+ mice at 5 ( J ), 7 ( K ) and 14 ( L ) dpi. Scale bar, 50 μm. Data is presented as mean ± SD. All P values were determined by unpaired Student’s t test.

    Journal: The EMBO Journal

    Article Title: GNAS/PKA signaling promotes aberrant osteochondral differentiation of Gli1 + tendon sheath progenitors

    doi: 10.1038/s44318-025-00553-7

    Figure Lengend Snippet: ( A – D ) Representative immunofluorescence images of GNAS expression in uninjured tendons ( A ) and injured sites of Gli1 - CreER T2 ; Ai9 mice at 5 ( B ), 7 ( C ) and 14 ( D ) dpi. Scale bar, 50 μm. ( E , F ) Statistical analysis of GNAS + region ( E ) and GNAS + /GLI1 + region ( F ) in uninjured tendons and injured sites of Gli1 - CreER T2 ; Acvr1 R206H/+ mice at 5, 7 and 14 dpi ( n = 5 per group). P values from left to right: **** P = 8.29e-10, **** P = 1.16e-8, **** P = 1.57e-8. **** P = 2.36e-9, **** P = 1.71e-6, **** P = 9.06e-6. ( G , H ) Statistical analysis of p-PKA substrate + region ( G ) and p-PKA substrate + /GLI1 + region ( H ) in uninjured tendons and injured sites of Gli1 - CreER T2 ; Acvr1 R206H/+ mice at 5, 7 and 14 dpi ( n = 5 per group). P values from left to right: **** P = 1.42e-6, **** P = 7.23e-6, **** P = 1.53e-5. **** P = 1.96e-5, ** P = 2.63e-3, **** P = 6.44e-4. ( I – L ) Representative immunofluorescence images of p-PKA substrate expression in uninjured tendons ( I ) and injured sites of Gli1 - CreER T2 ; Acvr1 R206H/+ mice at 5 ( J ), 7 ( K ) and 14 ( L ) dpi. Scale bar, 50 μm. Data is presented as mean ± SD. All P values were determined by unpaired Student’s t test.

    Article Snippet: Anti-mouse GNAS , Proteintech , Cat#10150-2-AP.

    Techniques: Immunofluorescence, Expressing

    ( A ) Representative HE staining showing the morphology of human tendon. Scale bar, 200 or 50 μm. ( B ) Representative immunofluorescence staining of GLI1 in human tendon. Scale bar, 10 μm. ( C ) Representative HE staining showing the morphology of HO from Ossification of the Posterior Longitudinal Ligament patients. Scale bar, 200 or 50 μm. ( D ) Representative immunofluorescence staining of GLI1 in HO from Ossification of the Posterior Longitudinal Ligament patients. Scale bar, 10 μm. ( E ) Statistical analysis of the GLI1 + cells in human normal tendon and HO lesion ( n = 3 per group). **** P = 7.88e-7. ( F , G ) Representative immunofluorescence staining ( F ) and statistical analysis ( G ) of GNAS in naive or chondrogenic tendon stem cells with or without NF449 treatment. ( n = 5 per group). **** P = 2.8e-14. Scale bar, 10 μm. ( H , I ) Representative immunofluorescence staining ( H ) and statistical analysis ( I ) of p-PKA substrate in naive or chondrogenic tendon stem cells with or without NF449 treatment. ( n = 5 per group). Data are represented as the mean ± SD. **** P = 9.77e-9. Scale bar, 10 μm. ( J , K ) Statistical analysis of SOX9 ( J , **** P = 8.91e-8) and RUNX2 ( K , **** P = 6.62e-6) in chondrogenic or osteogenic tendon stem cells with or without NF449 treatment. ( n = 6 per group). Data is presented as mean ± SD. All P values were determined by unpaired Student’s t test ( E ) and one-way ANOVA with Bonferroni post hoc test ( G , I , J , K ).

    Journal: The EMBO Journal

    Article Title: GNAS/PKA signaling promotes aberrant osteochondral differentiation of Gli1 + tendon sheath progenitors

    doi: 10.1038/s44318-025-00553-7

    Figure Lengend Snippet: ( A ) Representative HE staining showing the morphology of human tendon. Scale bar, 200 or 50 μm. ( B ) Representative immunofluorescence staining of GLI1 in human tendon. Scale bar, 10 μm. ( C ) Representative HE staining showing the morphology of HO from Ossification of the Posterior Longitudinal Ligament patients. Scale bar, 200 or 50 μm. ( D ) Representative immunofluorescence staining of GLI1 in HO from Ossification of the Posterior Longitudinal Ligament patients. Scale bar, 10 μm. ( E ) Statistical analysis of the GLI1 + cells in human normal tendon and HO lesion ( n = 3 per group). **** P = 7.88e-7. ( F , G ) Representative immunofluorescence staining ( F ) and statistical analysis ( G ) of GNAS in naive or chondrogenic tendon stem cells with or without NF449 treatment. ( n = 5 per group). **** P = 2.8e-14. Scale bar, 10 μm. ( H , I ) Representative immunofluorescence staining ( H ) and statistical analysis ( I ) of p-PKA substrate in naive or chondrogenic tendon stem cells with or without NF449 treatment. ( n = 5 per group). Data are represented as the mean ± SD. **** P = 9.77e-9. Scale bar, 10 μm. ( J , K ) Statistical analysis of SOX9 ( J , **** P = 8.91e-8) and RUNX2 ( K , **** P = 6.62e-6) in chondrogenic or osteogenic tendon stem cells with or without NF449 treatment. ( n = 6 per group). Data is presented as mean ± SD. All P values were determined by unpaired Student’s t test ( E ) and one-way ANOVA with Bonferroni post hoc test ( G , I , J , K ).

    Article Snippet: Anti-mouse GNAS , Proteintech , Cat#10150-2-AP.

    Techniques: Staining, Immunofluorescence